Pontificia Universidad Católica de Chile Pontificia Universidad Católica de Chile
Escudero C., Vera M., Oggerin M. and Amils R. (2018)

Active microbial biofilms in deep poor porous continental subsurface rocks

Revista : Scientific Reports
Volumen : 8
Páginas : 9 pp
Tipo de publicación : ISI Ir a publicación


Deep continental subsurface is defined as oligotrophic environments where microorganisms present a very low metabolic rate. To date, due to the energetic cost of production and maintenance of biofilms, their existence has not been considered in poor porous subsurface rocks. We applied fluorescence in situ hybridization techniques and confocal laser scanning microscopy in samples from a continental deep drilling project to analyze the prokaryotic diversity and distribution and the possible existence of biofilms. Our results show the existence of natural microbial biofilms at all checked depths of the Iberian Pyrite Belt (IPB) subsurface and the co-occurrence of bacteria and archaea in this environment. This observation suggests that multi-species biofilms may be a common and widespread lifestyle in subsurface environments.