Pontificia Universidad Católica de Chile Pontificia Universidad Católica de Chile
Sastre F., Ferreira F. and Pedreschi F. (2017)

A systematic approach for the chromatographic fractionation and purification of major steroid saponins in commercial extracts of Yucca schidigera Roezl

Revista : Journal of Chromatography B-Analytical Technologies in The Biomedical and Life Sciences
Volumen : 1046
Páginas : 235–242
Tipo de publicación : ISI Ir a publicación


Yucca schidigera Roezl. (yucca) is one of the major industrial sources of steroid saponins used as animal and human food additives. This work describes a new, systematic and reproducible three-step method by medium and high-pressure liquid chromatography (under RP, NP and RP conditions), for the isolation and purification of three groups of saponins, which were further purified in six sub-fractions, and finally into twelve individual steroid saponins previously reported in Y. schidigera. In accordance to the increasing applications of yucca extracts, further analytical, biological and physicochemical studies are still required. The presented method is applicable to the preparation of steroids saponins previously reported in commercial extracts of Y. schidigera, both as highly purified mixtures of defined composition, including twelve pure components.